The following technical application briefs demonstrate some of the capabilities of the cellTRAY Imaging System Model CT-1000. For specific assay and research questions, please contact us.

Mitochondrial Potential JC-1 Cell Viability Assay

JC-1 is a cationic dye that is used as an indicator of mitochondrial potential in cells. This application brief details a JC-1 cell viability assay, demonstrating the capabilities of the cellTRAY Imaging System for precise live cell containment, microfluidic delivery, automated high content acquisition and time-lapse imaging. A summary of methods and protocols, and experimental results obtained are also provided.

View Application Brief (PDF, 143KB)

Trypan Blue Cell Viability Assay

Trypan Blue is a stain commonly used to differentiate viable and nonviable cells. This application brief discusses the importance of Trypan Blue and cell viability assays in increasing our understanding of normal and defective apoptotic processes; and the development of a protocol for performing a Trypan Blue-based viability assay on drosophila KC cells treated with Valinomycin, using the cellTRAY Imaging System CT-1000.

View Application Brief (PDF, 77KB)

Bio-sensor Solutions using the cellTRAY with PortArray Microarray Reader
Collaboration with Aurora Photonics

Nanopoint and Aurora Photonics have teamed together to leverage the benefits of the Nanopoint's microarray cellTRAY Slide with Aurora Photonics portable bench-top scanner, the PortArray™ 5000. This powerful combination of miniaturized volumes, portability, and precision leads the way in field-based analysis readers.

Read More (PDF, 184KB)

HCS with sub-wavelength optical imaging of cells transferred acoustically into sub-microliter wells.
Collaboration with Labcyte, Inc.

Current methods of optical high content screening (HCS) involve cells cultured in Petri dishes, 96, 384 and 1536 well plates and on microscope slides. The costs of running many experiments in microtiter plates is high due to the amount of cell culture media, the number of cells required, and the amount of reagent used. This poster describes the use of Nanopoint's cellTRAY as a high-density cell containment HCS device and the loading of live cells employing acoustic droplet ejection (ADE) technology with Labcyte's Portrait™ 630 spotter.

View Poster (PDF, 105KB)

Cardiac Myocyte Cells in the cellTRAY

See a video of cultured cardiac cells on a Cell Matrix Chip Sensor for chem-bio toxins being developed by Cellular Bioengineering, Inc. in Honolulu, Hawaii.

View/Download Video (WMV, 7.9MB)